Enrichment of miR-17-5p enhances the protective effects of EPC-EXs on vascular and skeletal muscle injury in a diabetic hind limb ischemia model.

ABSTRACT:

Background/aims

Diabetes mellitus (DM) is highly susceptible to diabetic hind limb ischemia (DHI). MicroRNA (MiR)-17-5p is downregulated in DM and plays a key role in vascular protection. Endothelial progenitor cell (EPC)-released exosomes (EPC-EXs) contribute to vascular protection and ischemic tissue repair by transferring their contained miRs to target cells. Here, we investigated whether miR-17-5p-enriched EPC-EXs (EPC-EXs^miR-17-5p) had conspicuous effects on protecting vascular and skeletal muscle in DHI in vitro and in vivo.

Methods

EPCs transfected with scrambled control or miR-17-5p mimics were used to generate EPC-EXs and EPC-EXs^miR-17-5p. Db/db mice were subjected to hind limb ischemia. After the surgery, EPC-EXs and EPC-EXs^miR-17-5p

SUBMITTER: Pan Q 

PROVIDER: S-EPMC10067242 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature