FTO-mediated m6A demethylation of pri-miR-3591 alleviates osteoarthritis progression.

ABSTRACT:

Objectives

Increasing evidence have demonstrated the N6-methyladenosine (m⁶A) plays critical roles in osteoarthritis (OA) progression, but the role of m⁶A in OA has not been completely illuminated. Herein, we investigated the function and underlying mechanism of m⁶A demethylase fat mass and obesity-associated protein (FTO) in OA progression.

Materials and methods

The FTO expression was detected in mice OA cartilage tissues and lipopolysaccharide (LPS)-stimulated chondrocytes. Gain-of-function assays was used to evaluate the role of FTO in OA cartilage injury in vitro and in vivo. The miRNA-sequencing, RNA-binding protein immunoprecipitation (RIP), luciferase reporter assay, and in vitro pri-miRNA processing assays were conducted to confirm th

SUBMITTER: Liu W 

PROVIDER: S-EPMC10067311 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature