Delivery of miR-3529-3p using MnO₂ -SiO₂ -APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A.

ABSTRACT:

Background

The present study aimed to investigate the function of miR-3529-3p in lung adenocarcinoma and MnO₂ -SiO₂ -APTES (MSA) as a promising multifunctional delivery agent for lung adenocarcinoma therapy.

Methods

Expression levels of miR-3529-3p were evaluated in lung carcinoma cells and tissues by qRT-PCR. The effects of miR-3529-3p on apoptosis, proliferation, metastasis and neovascularization were assessed by CCK-8, FACS, transwell and wound healing assays, tube formation and xenografts experiments. Luciferase reporter assays, western blot, qRT-PCR and mitochondrial complex assay were used to determine the targeting relationship between miR-3529-3p and hypoxia-inducible gene domain family member 1A (HIGD1A). MSA was fabricated using MnO₂

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10067359 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature