Establishing pteridine metabolism in a progressive isogenic breast cancer cell model.

ABSTRACT:

Introduction

Pteridines include folate-derived metabolites that have been putatively associated with certain cancers in clinical studies. However, their biological significance in cancer metabolism and role in cancer development and progression remains poorly understood.

Objectives

The purpose of this study was to examine the effects of tumorigenicity on pteridine metabolism by studying a panel of 15 pteridine derivatives using a progressive breast cancer cell line model with and without folic acid dosing.

Methods

The MCF10A progressive breast cancer model, including sequentially derived MCF10A (benign), MCF10AT (premalignant), and MCF10CA1a (malignant) cell lines were dosed with 0, 100, and 250 mg/L folic acid. Pteridines were analyzed in both intracellular and extra

SUBMITTER: Rasmussen L 

PROVIDER: S-EPMC10067363 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature