Identification of candidate genes for nicotine withdrawal in C57BL/6J × DBA/2J recombinant inbred mice.

ABSTRACT: Nicotine is the reinforcing ingredient in tobacco. Following chronic exposure, sudden cessation of nicotine use produces negative symptoms of withdrawal that contribute to dependence. The molecular mechanisms underlying nicotine withdrawal behaviors, however, are poorly understood. Using recombinant inbred mice, chronic nicotine was delivered by minipump and withdrawal induced using mecamylamine. Somatic signs of withdrawal, and anxiety-like behavior using elevated plus maze, were then assessed. Interval mapping was used to identify associations between genetic variation and withdrawal behaviors, and with basal gene expression. Differential gene expression following nicotine exposure and withdrawal was also assessed in progenitor mice using microarrays. Quantitative trait loci mapping iden

SUBMITTER: Smith ML 

PROVIDER: S-EPMC10067406 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature