Single-cell next-generation sequencing of circulating tumor cells in patients with neuroblastoma.

ABSTRACT: Circulating tumor cells (CTCs) derived from any tumor tissue could contribute to metastasis and resistance to cancer treatments. In this study, we performed single-cell next-generation sequencing of CTCs and evaluated their usefulness for characterizing tumor biology and the mechanisms of metastasis in neuroblastomas (NB). We aimed to isolate CTCs from 10 patients with NB at diagnosis before any treatments and four patients at relapse. GD2⁺ CD90⁺ CD45^- CD235a^- DAPI^- cells were isolated as neuroblastoma CTCs using fluorescence-activated cell sorting. In five patients with advanced stages (M stage), DNA and RNA sequencing of CTCs at single-cell level were performed. NB CTCs were isolated from eight of the 10 patients at diagnosis and thr

SUBMITTER: Kojima M 

PROVIDER: S-EPMC10067419 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature