Chemical reprogramming of melanocytes to skeletal muscle cells.

ABSTRACT:

Background

Direct cell-fate conversion by chemical reprogramming is promising for regenerative cell therapies. However, this process requires the reactivation of a set of master transcription factors (TFs) of the target cell type, which has proven challenging using only small molecules.

Methods

We developed a novel small-molecule cocktail permitting robust skin cell to muscle cell conversion. By single cell sequencing analysis, we identified a Pax3 (Paired box 3)-expressing melanocyte population holding a superior myogenic potential outperforming other seven types of skin cells. We further validated the single cell sequencing analysis results using immunofluorescence staining, in situ hybridization and FACS sorting and confirmed the myogenic potential of melanocytes during c

SUBMITTER: Yang W 

PROVIDER: S-EPMC10067486 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature