High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples.

ABSTRACT: Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the 'Fast Green' dye that provides unmatched visualization of collagen 3D network architecture, via confocal or light-sheet microscopy, compatible with solvent-based tissue clearing and immunostaining.

SUBMITTER: Timin G 

PROVIDER: S-EPMC10067766 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature