Suprachoroidal Delivery in Rats and Guinea Pigs Using a High-Precision Microneedle Injector.

ABSTRACT:

Purpose

Methods of injection into the suprachoroidal space (SCS) have been developed for larger animals and humans, but reliable administration to the SCS of rodents remains challenging given their substantially smaller eyes. Here, we developed microneedle (MN)-based injectors for SCS delivery in rats and guinea pigs.

Methods

We optimized key design features, including MN size and tip characteristics, MN hub design, and eye stabilization, to maximize injection reliability. Performance of the injection technique was characterized in rats (n = 13) and guinea pigs (n = 3) in vivo using fundoscopy and histological examinations to validate targeted SCS delivery.

Results

To enable SCS injection across the thin rodent sclera, the injector featured an ultrasmall, hollow MN me

SUBMITTER: Hejri A 

PROVIDER: S-EPMC10067776 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature