The immune cell landscape and response of Marek's disease resistant and susceptible chickens infected with Marek's disease virus.

ABSTRACT: Genetically resistant or susceptible chickens to Marek's disease (MD) have been widely used models to identify the molecular determinants of these phenotypes. However, these prior studies lacked the basic identification and understanding of immune cell types that could be translated toward improved MD control. To gain insights into specific immune cell types and their responses to Marek's disease virus (MDV) infection, we used single-cell RNA sequencing (scRNAseq) on splenic cells from MD resistant and susceptible birds. In total, 14,378 cells formed clusters that identified various immune cell types. Lymphocytes, specifically T cell subtypes, were the most abundant with significant proportional changes in some subtypes upon infection. The largest number of differentially expressed genes (

SUBMITTER: Warren WC 

PROVIDER: S-EPMC10067856 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature