Protective effects of melatonin on myocardial microvascular endothelial cell injury under hypertensive state by regulating Mst1.

ABSTRACT:

Background

This study explored the protective effects of melatonin on the hypertensive model in myocardial microvascular endothelial cells.

Methods

Mouse myocardial microvascular endothelial cells were intervened with angiotensin II to establish hypertensive cell model and divided into control, hypertension (HP), hypertension + adenovirus negative control (HP + Ad-NC), hypertension + adenovirus carrying Mst1 (HP + Ad-Mst1), hypertension + melatonin (HP + MT), hypertension + adenovirus negative control + melatonin (HP + Ad-NC + MT), and hypertension + adenovirus carrying Mst1 + melatonin (HP + Ad-Mst1 + MT) groups. Autophagosomes were observed by transmission electron microscope. Mitochondrial membrane potential was detected by JC-1 staining. Apoptosis was detected by flow cy

SUBMITTER: Wang L 

PROVIDER: S-EPMC10068162 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature