Orexinergic innervations at GABAergic neurons of the lateral habenula mediates the anesthetic potency of sevoflurane.

ABSTRACT:

Aims

The circuitry mechanism associated with anesthesia-induced unconsciousness is still largely unknown. It has been reported that orexinergic neurons of the lateral hypothalamus (LHA) facilitate the emergence from anesthesia through their neuronal projections to the arousal-promoting brain areas. However, the lateral habenula (LHb), as one of the orexin downstream targets, is known for its anesthesia-promoting effect. Therefore, the current study aimed to explore whether and how the orexinergic projections from the LHA to the LHb have a regulatory effect on unconsciousness induced by general anesthesia.

Methods

We applied optogenetic, chemogenetic, or pharmacological approaches to regulate the orexinergic^LHA-LHb pathway. Fiber photometry was used to assess neuro

SUBMITTER: Zhou F 

PROVIDER: S-EPMC10068468 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature