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A simple method to make, trap and deform a vesicle in a gel.

ABSTRACT: We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of [Formula: see text]-Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non-intrusively, by indenting the surface of the gel.

SUBMITTER: Tapie P

PROVIDER: S-EPMC10068607 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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A simple method to make, trap and deform a vesicle in a gel.

Tapie Pierre P Prevost Alexis M AM Montel Lorraine L Pontani Léa-Laetitia LL Wandersman Elie E

Scientific reports 20230402 1

We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of [Formula: see text]-Hemolysin transmembrane proteins. Finally, w ...[more]

PMID: 37009808

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