M⁶A methyltransferase METTL3 programs CD4⁺ T-cell activation and effector T-cell differentiation in systemic lupus erythematosus.

ABSTRACT:

Background

Systemic lupus erythematosus (SLE) is an autoimmune disorder in which excessive CD4⁺ T-cell activation and imbalanced effector T-cell differentiation play critical roles. Recent studies have implied a potential association between posttranscriptional N6-methyladenosine (m⁶A) modification and CD4⁺ T-cell-mediated humoral immunity. However, how this biological process contributes to lupus is not well understood. In this work, we investigated the role of the m⁶A methyltransferase like 3 (METTL3) in CD4⁺ T-cell activation, differentiation, and SLE pathogenesis both in vitro and in vivo.

Methods

The expression of METTL3 was knocked down and METTL3 enzyme activity was inhibited using siRNA and catalytic inhibitor, r

SUBMITTER: Lu S 

PROVIDER: S-EPMC10068720 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature