Two-point optical manipulation reveals mechanosensitive remodeling of cell-cell contacts in vivo.

ABSTRACT: Biological tissues acquire reproducible shapes during development through dynamic cell behaviors. Most of these behaviors involve the remodeling of cell-cell contacts. During epithelial morphogenesis, contractile actomyosin networks remodel cell-cell contacts by shrinking and extending junctions between lateral cell surfaces. However, actomyosin networks not only generate mechanical stresses but also respond to them, confounding our understanding of how mechanical stresses remodel cell-cell contacts. Here, we develop a two-point optical manipulation method to impose different stress patterns on cell-cell contacts in the early epithelium of the Drosophila embryo. The technique allows us to produce junction extension and shrinkage through different push and pull manipulations at the e

SUBMITTER: Nishizawa K 

PROVIDER: S-EPMC10068846 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature