Identification of Conserved and Novel MicroRNAs with their Targets in Garden Pea (Pisum Sativum L.) Leaves by High-Throughput Sequencing.

ABSTRACT: MicroRNAs (miRNAs) are single-stranded, endogenous, non-coding RNAs of 20-24 nucleotides that play a significant role in post-transcriptional gene regulation. Various conserved and novel miRNAs have been characterized, especially from the plant species whose genomes were well-characterized; however, information on miRNA in economically important plants such as pea (Pisum sativum L.) is limited. In this study, I have identified conserved and novel miRNA in garden pea plant leaves samples along with their targets by analyzing the next generation sequencing (NGS) data. The raw data obtained from NGS were processed and 1.38 million high-quality non-redundant reads were retained for analysis, this tremendous quantity of reads indicates a large and diverse small RNA population in pea leav

SUBMITTER: Khan QH 

PROVIDER: S-EPMC10068972 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature