HMSH2 coordinated with the expression of E2F1 promotes platinum response in epithelial ovarian cancer.

ABSTRACT:

Objective

To explore underlying mechanisms that regulate hMSH2 expression and drug susceptibility in epithelial ovarian cancer (EOC).

Methods

Using data from the Cancer Genome Atlas (TCGA) we used bioinformatical analysis to predict transcription factors (TFs) that potentially regulate hMSH2. RT-qPCR, Western blot, and luciferase assays were undertaken using ovarian cancer cell lines to verify the identified TF. Expressions of the TF were modulated using overexpression or knockdown, and the corresponding cellular responses to cisplatin were examined.

Results

The TF, E2F1, was found to regulate the hMSH2 gene. The expression level of E2F1 correlated with cisplatin susceptibility in vitro. Kaplan-Meier analysis of 77 patients with EOC showed that low E2F1 express

SUBMITTER: Hu XQ 

PROVIDER: S-EPMC10068988 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature