PurificationDB: database of purification conditions for proteins.

ABSTRACT: The isolation of proteins of interest from cell lysates is an integral step to study protein structure and function. Liquid chromatography is a technique commonly used for protein purification, where the separation is performed by exploiting the differences in physical and chemical characteristics of proteins. The complex nature of proteins requires researchers to carefully choose buffers that maintain stability and activity of the protein while also allowing for appropriate interaction with chromatography columns. To choose the proper buffer, biochemists often search for reports of successful purification in the literature; however, they often encounter roadblocks such as lack of accessibility to journals, non-exhaustive specification of components and unfamiliar naming conventions. To ov

SUBMITTER: Garland O 

PROVIDER: S-EPMC10069378 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature