Visualizing in situ viral replication across the natural history of chronic HBV infection.

ABSTRACT:

Background and aims

Chronic HBV infection evolves through different phases. Interactions between viral replication and the host immune response in the liver underlie the pathogenesis of this disease. The aim of this study was to directly visualize the HBV replication intermediates at a single-cell resolution inscribed on morphological changes corresponding to disease activity.

Methods

A set of archived formalin-fixed paraffin-embedded liver needle biopsies from treatment-naïve patients were collected and categorized into phases according to the American Association for the Study of the Liver Diseases (AASLD) guidelines. HBV RNA and DNA were detected using in situ hybridization assays.

Results

The hepatocytes were ubiquitously infected in subjects with immune tolerance

SUBMITTER: Zhang H 

PROVIDER: S-EPMC10069836 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature