Phenotypically distinguishing ESBL-producing pathogens using paper-based surface enhanced Raman sensors.

ABSTRACT: Antimicrobial stewardship practices are critical in preventing the further erosion of treatment options for bacterial infections. Yet, at the same time, determination of an infection's antimicrobial susceptibility requires multiple rounds of culture and expensive lab automation systems. In this work, we report the use of paper-based surface enhanced Raman spectroscopy (SERS) sensors and portable instrumentation to phenotypically discriminate multi-drug resistance with fewer culture steps than conventional clinical microbiology. Specifically, we demonstrate the identification of resistance to varying generations of β-lactam antibiotics by detecting the activity of particular β-lactamase enzymes in a multiplexed assay. The method utilizes molecular reporters that consist of β-lactams with SE

SUBMITTER: Hilton SH 

PROVIDER: S-EPMC10069952 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature