Tumour-educated platelets for breast cancer detection: biological and technical insights.

ABSTRACT:

Background

Studies have shown that blood platelets contain tumour-specific mRNA profiles tumour-educated platelets (TEPs). Here, we aim to train a TEP-based breast cancer detection classifier.

Methods

Platelet mRNA was sequenced from 266 women with stage I-IV breast cancer and 212 female controls from 6 hospitals. A particle swarm optimised support vector machine (PSO-SVM) and an elastic net-based classifier (EN) were trained on 71% of the study population. Classifier performance was evaluated in the remainder (29%) of the population, followed by validation in an independent set (37 cases and 36 controls). Potential confounding was assessed in post hoc analyses.

Results

Both classifiers reached an area under the curve (AUC) of 0.85 upon internal validation. Reproducib

SUBMITTER: Liefaard MC 

PROVIDER: S-EPMC10070267 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature