Retinoic acid signaling modulation guides in vitro specification of human heart field-specific progenitor pools.

ABSTRACT: Cardiogenesis relies on the precise spatiotemporal coordination of multiple progenitor populations. Understanding the specification and differentiation of these distinct progenitor pools during human embryonic development is crucial for advancing our knowledge of congenital cardiac malformations and designing new regenerative therapies. By combining genetic labelling, single-cell transcriptomics, and ex vivo human-mouse embryonic chimeras we uncovered that modulation of retinoic acid signaling instructs human pluripotent stem cells to form heart field-specific progenitors with distinct fate potentials. In addition to the classical first and second heart fields, we observed the appearance of juxta-cardiac field progenitors giving rise to both myocardial and epicardial cells. Applying these

SUBMITTER: Zawada D 

PROVIDER: S-EPMC10070453 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature