Drug cytotoxicity screening using human intestinal organoids propagated with extensive cost-reduction strategies.

ABSTRACT: Organoids are regarded as physiologically relevant cell models and useful for compound screening for drug development; however, their applications are currently limited because of the high cost of their culture. We previously succeeded in reducing the cost of human intestinal organoid culture using conditioned medium (CM) of L cells co-expressing Wnt3a, R-spondin1, and Noggin. Here, we further reduced the cost by replacing recombinant hepatocyte growth factor with CM. Moreover, we showed that embedding organoids in collagen gel, a more inexpensive matrix than Matrigel, maintains organoid proliferation and marker gene expression similarly when using Matrigel. The combination of these replacements also enabled the organoid-oriented monolayer cell culture. Furthermore, screening thousands of

SUBMITTER: Takahashi Y 

PROVIDER: S-EPMC10070462 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature