Structural mass spectrometry approaches to understand multidrug efflux systems.

ABSTRACT: Multidrug efflux pumps are ubiquitous across both eukaryotes and prokaryotes, and have major implications in antimicrobial and multidrug resistance. They reside within cellular membranes and have proven difficult to study owing to their hydrophobic character and relationship with their compositionally complex lipid environment. Advances in structural mass spectrometry (MS) techniques have made it possible to study these systems to elucidate critical information on their structure-function relationships. For example, MS techniques can report on protein structural dynamics, stoichiometry, connectivity, solvent accessibility, and binding interactions with ligands, lipids, and other proteins. This information proving powerful when used in conjunction with complementary structural biology metho

SUBMITTER: Russell Lewis B 

PROVIDER: S-EPMC10070475 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature