WSSV exploits AMPK to activate mTORC2 signaling for proliferation by enhancing aerobic glycolysis.

ABSTRACT: AMPK plays significant roles in the modulation of metabolic reprogramming and viral infection. However, the detailed mechanism by which AMPK affects viral infection is unclear. The present study aims to determine how AMPK influences white spot syndrome virus (WSSV) infection in shrimp (Marsupenaeus japonicus). Here, we find that AMPK expression and phosphorylation are significantly upregulated in WSSV-infected shrimp. WSSV replication decreases remarkably after knockdown of Ampkα and the shrimp survival rate of AMPK-inhibitor injection shrimp increases significantly, suggesting that AMPK is beneficial for WSSV proliferation. Mechanistically, WSSV infection increases intracellular Ca²⁺ level, and activates CaMKK, which result in AMPK phosphorylation and partial nuclear translocat

SUBMITTER: Zhang P 

PROVIDER: S-EPMC10070494 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature