Isolation and identification of extracellular matrix proteins from oil-based CASPERized mouse brains for matrisomal analysis.

ABSTRACT: The extracellular matrix (ECM) components present within all tissues and organs help to maintain the cytoskeletal architecture and tissue morphology. Although the ECM plays a role in cellular events and signaling pathways, it has not been well studied due its insolubility and complexity. Brain tissue has a higher cell density and weaker mechanical strength than other tissues in the body. When removing cells using a general decellularization method to produce scaffolds and obtain ECM proteins, various problems must be considered because tissues are easily damaged. To retain the brain shape and ECM components, we performed decellularization in combination with polymerization. We immersed mouse brains in oil for polymerization and decellularization via O-CASPER (Oil-based Clinically and Exper

SUBMITTER: Ha BG 

PROVIDER: S-EPMC10070542 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature