Distinct transcriptomic and epigenomic modalities underpin human memory T cell subsets and their activation potential.

ABSTRACT: Human memory T cells (MTC) are poised to rapidly respond to antigen re-exposure. Here, we derived the transcriptional and epigenetic programs of resting and ex vivo activated, circulating CD4⁺ and CD8⁺ MTC subsets. A progressive gradient of gene expression from naïve to T_CM to T_EM is observed, which is accompanied by corresponding changes in chromatin accessibility. Transcriptional changes suggest adaptations of metabolism that are reflected in altered metabolic capacity. Other differences involve regulatory modalities comprised of discrete accessible chromatin patterns, transcription factor binding motif enrichment, and evidence of epigenetic priming. Basic-helix-loop-helix factor motifs for AHR and HIF1A distinguish subsets and predict transcri

SUBMITTER: Rose JR 

PROVIDER: S-EPMC10070634 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature