A flagellin-conjugate protein induces dual NLRC4- and NLRP3-inflammasome activation which modulates inflammatory cytokine secretion from macrophages.

ABSTRACT:

Background

A recombinant fusion protein combining the adjuvant and TLR5-ligand flagellin with the major birch pollen allergen Bet v 1 (rFlaA:Betv1) has been suggested to prevent the manifestation of birch allergy. Noteworthy, rFlaA:Betv1 induced both pro- and anti-inflammatory responses which were differentially regulated. However, the mechanism by which flagellin fusion proteins modulate allergen-specific immune responses, especially the mechanisms underlying IL-1β secretion and their contribution to the overall immune responses remains elusive.

Objective

To investigate the mechanisms underlying the production of IL-1β from rFlaA:Betv1 stimulated macrophages.

Methods

Macrophages were derived from mouse peritoneal-, human buffy-coat-, and PMA-differentiated THP-1 (wil

SUBMITTER: Lin YJ 

PROVIDER: S-EPMC10070734 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature