AGEs-RAGE-K_Ca3.1 pathway mediates palmitic acid-induced migration of PBMCs from patients with type 2 diabetes.

ABSTRACT: Type 2 diabetes mellitus (T2DM) is characterized by chronic low-grade systemic inflammation. Tissue infiltration by monocyte migration contributes to the pathogenesis of vascular complications in T2DM. We studied the role of intermediate-conductance Ca²⁺-activated K⁺ (K_Ca3.1) channels in the palmitic acid (PA)-induced migration of peripheral blood mononuclear cells (PBMCs) from T2DM patients and the influence of advanced glycation endproducts (AGEs). A total of 49 T2DM patients and 33 healthy subjects was recruited into this study. Using flow cytometry and Western blotting analysis as well as cell migration assay, we found that there was a significant decrease in frequency of T lymphocytes and monocytes in CD45⁺ leukocyte population. PA at 100 μM

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10070889 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature