Dataset Information

Antioxidant effect of ergothioneine on in vitro maturation of porcine oocytes.

ABSTRACT:

Background

Ergothioneine (EGT) is a natural amino acid derivative in various animal organs and is a bioactive compound recognized as a food and medicine.

Objectives

This study examined the effects of EGT supplementation during the in vitro maturation (IVM) period on porcine oocyte maturation and subsequent embryonic development competence after in vitro fertilization (IVF).

Methods

Each EGT concentration (0, 10, 50, and 100 µM) was supplemented in the maturation medium during IVM. After IVM, nuclear maturation, intracellular glutathione (GSH), and reactive oxygen species (ROS) levels of oocytes were investigated. In addition, the genes related to cumulus function and antioxidant pathways in oocytes or cumulus cells were investigated. Finally, this study examined whether EGT could affect embryonic development after IVF.

Results

After IVM, the EGT supplementation group showed significantly higher intracellular GSH levels and significantly lower intracellular ROS levels than the control group. Moreover, the expression levels of hyaluronan synthase 2 and Connexin 43 were significantly higher in the 10 µM EGT group than in the control group. The expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and NAD(P)H quinone dehydrogenase 1 (NQO1) were significantly higher in the oocytes of the 10 µM EGT group than in the control group. In the assessment of subsequent embryonic development after IVF, the 10 µM EGT treatment group improved the cleavage and blastocyst rate significantly than the control group.

Conclusions

Supplementation of EGT improved oocyte maturation and embryonic development by reducing oxidative stress in IVM oocytes.

SUBMITTER: Jeong JY

PROVIDER: S-EPMC10071278 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Publications

Antioxidant effect of ergothioneine on <i>in vitro</i> maturation of porcine oocytes.

Jeong Ji-Young JY Cai Lian L Kim Mirae M Choi Hyerin H Oh Dongjin D Jawad Ali A Kim Sohee S Zheng Haomiao H Lee Eunsong E Lee Joohyeong J Hyun Sang-Hwan SH

Journal of veterinary science 20230301 2

<h4>Background</h4>Ergothioneine (EGT) is a natural amino acid derivative in various animal organs and is a bioactive compound recognized as a food and medicine.<h4>Objectives</h4>This study examined the effects of EGT supplementation during the <i>in vitro</i> maturation (IVM) period on porcine oocyte maturation and subsequent embryonic development competence after <i>in vitro</i> fertilization (IVF).<h4>Methods</h4>Each EGT concentration (0, 10, 50, and 100 µM) was supplemented in the maturati ...[more]

PMID: 37012032

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