Isolation of feline panleukopenia virus from Yanji of China and molecular epidemiology from 2021 to 2022.

ABSTRACT:

Background

Feline panleukopenia virus (FPV) is a widespread and highly infectious pathogen in cats with a high mortality rate. Although Yanji has a developed cat breeding industry, the variation of FPV locally is still unclear.

Objectives

This study aimed to isolate and investigate the epidemiology of FPV in Yanji between 2021 and 2022.

Methods

A strain of FPV was isolated from F81 cells. Cats suspected of FPV infection (n = 80) between 2021 and 2022 from Yanji were enrolled in this study. The capsid protein 2 (VP2) of FPV was amplified. It was cloned into the pMD-19T vector and transformed into a competent Escherichia coli strain. The positive colonies were analyzed via VP2 Sanger sequencing. A phylogenetic analysis based on a VP2 coding sequence was performed

SUBMITTER: Xue H 

PROVIDER: S-EPMC10071280 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature