FYN/TOPK/HSPB1 axis facilitates the proliferation and metastasis of gastric cancer.

ABSTRACT:

Background

FYN is a nonreceptor tyrosine kinase that regulates diverse pathological processes. The pro-cancer role of FYN in multiple malignancies has been elucidated. However, the mechanisms that FYN promotes gastric cancer (GC) progression remain largely unknown.

Methods

In vitro and in vivo assays were used to investigate the function of FYN. FYN, TOPK, p-TOPK expression in GC specimens were detected by immunohistochemistry. Phosphoproteomics assays identify TOPK downstream substrate molecules. The molecular mechanism was determined using COIP assays, pull-down assays, immunofluorescence co-localization assays, western blotting, ³²p-labeled isotope radioautography assays, vitro kinase assays, and TOPK knockout mice.

Results

FYN was found to be significan

SUBMITTER: Peng S 

PROVIDER: S-EPMC10071617 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature