Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization.

ABSTRACT:

Background

Adaptation to alkalinization of the medium in fungi involves an extensive remodeling of gene expression. Komagataella phaffii is an ascomycetous yeast that has become an organism widely used for heterologous protein expression. We explore here the transcriptional impact of moderate alkalinization in this yeast, in search of suitable novel promoters able to drive transcription in response to the pH signal.

Results

In spite of a minor effect on growth, shifting the cultures from pH 5.5 to 8.0 or 8.2 provokes significant changes in the mRNA levels of over 700 genes. Functional categories such as arginine and methionine biosynthesis, non-reductive iron uptake and phosphate metabolism are enriched in induced genes, whereas many genes encoding iron-sulfur proteins or me

SUBMITTER: Albacar M 

PROVIDER: S-EPMC10071690 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature