Whole transcriptome analysis of platelet concentrates during storage.

ABSTRACT:

Background

Platelets are anucleated blood cells and contain various RNA species. We investigated the changes in the whole transcriptome expression profile of platelet concentrates (PC) during storage to explore biological functions and biomarkers in platelet storage damage.

Materials and methods

Platelets were collected by apheresis from eight healthy blood donors and stored from day 0 to day 4. Platelet phenotyping and function analysis were used to detect platelet activity during storage. RNA-sequencing was used to detect changes in expression of mRNA, lncRNA and circRNA in the PC during storage. Gene ontology and KEGG analyses were applied to predict the functional distribution of differential expression of mRNA. Gene set enrichment analysis was used to analyze the differ

SUBMITTER: Heililahong H 

PROVIDER: S-EPMC10072990 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature