ADAD2 functions in spermiogenesis and piRNA biogenesis in mice.

ABSTRACT:

Background

Adenosine deaminase domain containing 2 (ADAD2) is a testis-specific protein composed of a double-stranded RNA binding domain and a non-catalytic adenosine deaminase domain. A recent study showed that ADAD2 is indispensable for the male reproduction in mice. However, the detailed functions of ADAD2 remain elusive.

Objectives

This study aimed to investigate the cause of male sterility in Adad2 mutant mice and to understand the molecular functions of ADAD2.

Materials and methods

Adad2 homozygous mutant mouse lines, Adad2^-/- and Adad2^Δ/Δ , were generated by CRISPR/Cas9. Western blotting and immunohistochemistry were used to reveal the expression and subcellular localization of ADAD2. Co-immunoprecipitation tandem mass spectrometry was emp

SUBMITTER: Lu Y 

PROVIDER: S-EPMC10073342 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature