Third SARS-CoV-2 vaccination and breakthrough infections enhance humoral and cellular immunity against variants of concern.

ABSTRACT:

Introduction

SARS-CoV-2 vaccination is the leading strategy to prevent severe courses after SARS-CoV-2 infection. In our study, we analyzed humoral and cellular immune responses in detail to three consecutive homologous or heterologous SARS-CoV-2 vaccinations and breakthrough infections.

Methods

Peripheral blood samples of n=20 individuals were analyzed in the time course of three SARS-CoV-2 vaccinations and/or breakthrough infection. S1-, RBD-, S2- and N-specific IgG antibodies were quantified using Luminex-based multiplex assays and electrochemiluminescence multiplex assays for surrogate neutralization in plasma. Changes in cellular immune components were determined via flow cytometry of whole blood samples.

Results

All individuals (n=20) responded to vaccination wi

SUBMITTER: Ruhl L 

PROVIDER: S-EPMC10073596 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature