Temporal tracking of microglial and monocyte single-cell transcriptomics in lethal flavivirus infection.

ABSTRACT: As the resident parenchymal myeloid population in the central nervous system (CNS), microglia are strategically positioned to respond to neurotropic virus invasion and have been implicated in promoting both disease resolution and progression in the acute and post-infectious phase of virus encephalitis. In a mouse model of West Nile virus encephalitis (WNE), infection of the CNS results in recruitment of large numbers of peripheral immune cells into the brain, the majority being nitric oxide (NO)-producing Ly6C^hi inflammatory monocyte-derived cells (MCs). In this model, these cells enhance immunopathology and mortality. However, the contribution of microglia to this response is currently undefined. Here we used a combination of experimental tools, including single-cell RNA sequen

SUBMITTER: Spiteri AG 

PROVIDER: S-EPMC10074823 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature