Modeling of Ca²⁺ transients initiated by GPCR agonists in mesenchymal stromal cells.

ABSTRACT: The integrative study that included experimentation and mathematical modeling was carried out to analyze dynamic aspects of transient Ca²⁺ signaling induced by brief pulses of GPCR agonists in mesenchymal stromal cells from the human adipose tissue (AD-MSCs). The experimental findings argued for IP₃/Ca²⁺-regulated Ca²⁺ release via IP₃ receptors (IP₃Rs) as a key mechanism mediating agonist-dependent Ca²⁺ transients. The consistent signaling circuit was proposed to formalize coupling of agonist binding to Ca²⁺ mobilization for mathematical modeling. The model properly simulated the basic phenomenology of agonist transduction in AD-MSCs, which mostly produced single Ca²⁺ spikes upon brief stimul

SUBMITTER: Kaimachnikov NP 

PROVIDER: S-EPMC10074909 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature