N^ε-(carboxymethyl)lysine promotes lipid uptake of macrophage via cluster of differentiation 36 and receptor for advanced glycation end products.

ABSTRACT:

Background

Advanced glycation end products (AGEs) are diabetic metabolic toxic products that cannot be ignored. N^ε-(carboxymethyl)lysine (CML), a component of AGEs, could increase macrophage lipid uptake, promote foam cell formation, and thereby accelerate atherosclerosis. The receptor for AGEs (RAGE) and cluster of differentiation 36 (CD36) were the receptors of CML. However, it is still unknown whether RAGE and CD36 play key roles in CML-promoted lipid uptake.

Aim

Our study aimed to explore the role of RAGE and CD36 in CML-induced mac-rophage lipid uptake.

Methods

In this study, we examined the effect of CML on lipid uptake by Raw264.7 macrophages. After adding 10 mmol/L CML, the lipid accumulation in macro-phages was confirmed by oil red O staining. Expr

SUBMITTER: Wang ZQ 

PROVIDER: S-EPMC10075039 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature