Hydrogel armed with Bmp2 mRNA-enriched exosomes enhances bone regeneration.

ABSTRACT:

Background

Sustained release of bioactive BMP2 (bone morphogenetic protein-2) is important for bone regeneration, while the intrinsic short half-life of BMP2 at protein level cannot meet the clinical need. In this study, we aimed to design Bmp2 mRNA-enriched engineered exosomes, which were then loaded into specific hydrogel to achieve sustained release for more efficient and safe bone regeneration.

Results

Bmp2 mRNA was enriched into exosomes by selective inhibition of translation in donor cells, in which NoBody (non-annotated P-body dissociating polypeptide, a protein that inhibits mRNA translation) and modified engineered BMP2 plasmids were co-transfected. The derived exosomes were named Exo^BMP2+NoBody. In vitro experiments confirmed that Exo^BMP2+NoBody

SUBMITTER: Yang Z 

PROVIDER: S-EPMC10075167 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature