Use of the waxworm Galleria mellonella larvae as an infection model to study Acinetobacter baumannii.

ABSTRACT: Galleria mellonella larvae have been increasingly used in research, including microbial infection studies. They act as suitable preliminary infection models to study host-pathogen interactions due to their advantages, such as the ability to survive at 37°C mimicking human body temperature, their immune system shares similarities with mammalian immune systems, and their short life cycle allowing large-scale studies. Here, we present a protocol for simple rearing and maintenance of G. mellonella without requiring special instruments and specialized training. This allows the continuous supply of healthy G. mellonella for research purposes. Besides, this protocol also provides detailed procedures on the (i) G. mellonella infection assays (killing assay and bacterial burden assay) for virulence

SUBMITTER: Ten KE 

PROVIDER: S-EPMC10075412 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature