Evaluation of decellularization process for developing osteogenic bovine cancellous bone scaffolds in-vitro.

ABSTRACT: Current immunological issues in bone grafting regarding the transfer of xenogeneic donor bone cells into the recipient are challenging the industry to produce safer acellular natural matrices for bone regeneration. The aim of this study was to investigate the efficacy of a novel decellularization technique for producing bovine cancellous bone scaffold and compare its physicochemical, mechanical, and biological characteristics with demineralized cancellous bone scaffold in an in-vitro study. Cancellous bone blocks were harvested from a bovine femoral head (18-24 months old) subjected to physical cleansing and chemical defatting, and further processed in two ways. Group I was subjected to demineralization, while Group II underwent decellularization through physical, chemical, and enzymatic t

SUBMITTER: Al Qabbani A 

PROVIDER: S-EPMC10075422 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature