3D reconstruction of the cerebellar germinal layer reveals tunneling connections between developing granule cells.

ABSTRACT: The difficulty of retrieving high-resolution, in vivo evidence of the proliferative and migratory processes occurring in neural germinal zones has limited our understanding of neurodevelopmental mechanisms. Here, we used a connectomic approach using a high-resolution, serial-sectioning scanning electron microscopy volume to investigate the laminar cytoarchitecture of the transient external granular layer (EGL) of the developing cerebellum, where granule cells coordinate a series of mitotic and migratory events. By integrating image segmentation, three-dimensional reconstruction, and deep-learning approaches, we found and characterized anatomically complex intercellular connections bridging pairs of cerebellar granule cells throughout the EGL. Connected cells were either mitotic, migratory,

SUBMITTER: Cordero Cervantes D 

PROVIDER: S-EPMC10075961 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature