Senescent cell population with ZEB1 transcription factor as its main regulator promotes osteoarthritis in cartilage and meniscus.

ABSTRACT:

Objectives

Single-cell level analysis of articular cartilage and meniscus tissues from human healthy and osteoarthritis (OA) knees.

Methods

Single-cell RNA sequencing (scRNA-seq) analyses were performed on articular cartilage and meniscus tissues from healthy (n=6, n=7) and OA (n=6, n=6) knees. Expression of genes of interest was validated using immunohistochemistry and RNA-seq and function was analysed by gene overexpression and depletion.

Results

scRNA-seq analyses of human knee articular cartilage (70 972 cells) and meniscus (78 017 cells) identified a pathogenic subset that is shared between both tissues. This cell population is expanded in OA and has strong OA and senescence gene signatures. Further, this subset has critical roles in extracellular matrix (ECM) an

SUBMITTER: Swahn H 

PROVIDER: S-EPMC10076001 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature