A 3D Bioprinted Gut Anaerobic Model for Studying Bacteria-Host Interactions.

ABSTRACT: The role of the human intestinal tract in host-microbe interactions has been highlighted in recent years. Several 3-dimensional (3D) models have been developed to reproduce the physiological characteristics of the human gut and to investigate the function of the gut microbiota. One challenge for 3D models is to recapitulate the low oxygen concentrations in the intestinal lumen. Moreover, most earlier 3D culture systems used a membrane to physically separate bacteria from the intestinal epithelium, which has sometimes made the studies of bacteria adhering to or invading cells less feasible. We report the establishment of a 3D gut epithelium model and cultured it at high cell viability under an anaerobic condition. We further cocultured intestinal bacteria including both commensal and pathog

SUBMITTER: Cheng L 

PROVIDER: S-EPMC10076011 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature