Establishment of guinea pig kidney cell lines with potential application in the production of a classical swine fever live GPE^- vaccine.

ABSTRACT: Classical swine fever (CSF) live vaccine used in Japan, GPE^- strain, is produced using guinea pig kidney (GPK)-derived primary culture cells. This means that a large number of guinea pigs are used to generate the primary GPK cells needed to produce the CSF live vaccine, and alternative solution is desired. Hence, we established two GPK cell lines capable of culturing the GPE^- strain: spontaneously immortalized GPK (GPK-SI) cells were generated by repeated passaging of primary GPK cells, and the other cell line, artificially immortalized GPK (GPK-AI) cells, were obtained by introducing the SV40 large T antigen gene into primary GPK cells. Both cell lines were susceptible to the GPE^- virus, and the virus grew more efficiently in GPK-SI cells at 37°C. When the

SUBMITTER: Shioda M 

PROVIDER: S-EPMC10076188 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature