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Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease.

ABSTRACT: Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this study, we purified SARS-CoV-2 PLpro as an immune antigen, constructed a nanobody phage display library, and identified a set of nanobodies with high affinity for SARS-CoV-2. In addition, enzyme activity experiments demonstrated that two nanobodies had a significant inhibitory effect on the PLpro. These nanobodies should therefore be investigated as candidates for the treatment of coronaviruses.

SUBMITTER: Qiao H

PROVIDER: S-EPMC10076250 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease.

Qiao Huarui H Li Lingyun L Wang Lu L Yu Haijun H Hu Fangzheng F Zhou Xin X Yang Hongzhao H Xu Jianfeng J Meng Xiangjing X Geng Yong Y Dai Yuanyuan Y

Protein expression and purification 20230406

Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this study, we purified SARS-CoV-2 PLpro as an immune antigen, constructed a nanobody phage display library, and identified a set of nanobodies with high affinity for SARS-CoV-2. In addition, enzyme activity ...[more]

PMID: 37030644

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