Systematic comparison of tools used for m⁶A mapping from nanopore direct RNA sequencing.

ABSTRACT: N6-methyladenosine (m6A) has been increasingly recognized as a new and important regulator of gene expression. To date, transcriptome-wide m6A detection primarily relies on well-established methods using next-generation sequencing (NGS) platform. However, direct RNA sequencing (DRS) using the Oxford Nanopore Technologies (ONT) platform has recently emerged as a promising alternative method to study m6A. While multiple computational tools are being developed to facilitate the direct detection of nucleotide modifications, little is known about the capabilities and limitations of these tools. Here, we systematically compare ten tools used for mapping m6A from ONT DRS data. We find that most tools present a trade-off between precision and recall, and integrating results from multiple tools gre

SUBMITTER: Zhong ZD 

PROVIDER: S-EPMC10076423 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature