Bacterial expression systems based on Tymovirus-like particles for the presentation of vaccine antigens.

ABSTRACT: Virus-like particles (VLPs) are virus-derived artificial nanostructures that resemble a native virus-stimulating immune system through highly repetitive surface structures. Improved safety profiles, flexibility in vaccine construction, and the ease of VLP production and purification have highlighted VLPs as attractive candidates for universal vaccine platform generation, although exploration of different types of expression systems for their development is needed. Here, we demonstrate the construction of several simple Escherichia coli expression systems for the generation of eggplant mosaic virus (EMV) VLP-derived vaccines. We used different principles of antigen incorporation, including direct fusion of EMV coat protein (CP) with major cat allergen Feld1, coexpression of antigen c

SUBMITTER: Ogrina A 

PROVIDER: S-EPMC10076540 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature