CDetection.v2: One-pot assay for the detection of SARS-CoV-2.

ABSTRACT:

Introduction

The ongoing 2019 coronavirus disease pandemic (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants, is a global public health threat. Early diagnosis and identification of SARS-CoV-2 and its variants plays a critical role in COVID-19 prevention and control. Currently, the most widely used technique to detect SARS-CoV-2 is quantitative reverse transcription real-time quantitative PCR (RT-qPCR), which takes nearly 1 hour and should be performed by experienced personnel to ensure the accuracy of results. Therefore, the development of a nucleic acid detection kit with higher sensitivity, faster detection and greater accuracy is important.

Methods

Here, we optimized the system components and reaction conditions of our pr

SUBMITTER: Wang X 

PROVIDER: S-EPMC10076661 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature